Perinatal maternal application of Lactobacillus rhamnosus GG suppresses allergic airway inflammation in mouse offspring

摘要: Summary Background Clinical studies indicate that maternal exposure to probiotic bacteria may protect from the development of allergic disease later in life. Objective The purpose this study was analyse effects a perinatal Lactobacillus rhamnosus GG (LGG) supplementation on disorders offspring. Methods Female BALB/c mice received intragastric LGG every other day before conception, during pregnancy and lactation (perinatal group) or conception only (prenatal group). Cytokine expression placental tissues examined. Offspring LGG-supplemented sham-exposed mothers were sensitized Ovalbumin (OVA), followed by aerosol allergen challenges. Development experimental asthma assessed bronchoalveolar lavage analysis, lung histology function measurement. production splenic mononuclear cells analysed following vitro stimulation. Results Intestinal colonization with observed mother only, but not offspring. However, reduced TNF-α, IFN-γ, IL-5 as well IL-10 derived perinatally mothers, whereas IL-13 IL-4 remained unchanged. Moreover, offspring prenatally airway peribronchial inflammation goblet cell hyperplasia significantly compared non-supplemented mothers. In contrast, hyperresponsiveness methacholine affected. Exposure shifted cytokine pattern markedly increased TNF-α level. Conclusion Our data suggest exert beneficial asthma, when applied very early phase life. Immunological are, at least parts, mediated via placenta, probably induction pro-inflammatory signals.