Genetic diversity of bovine Neospora caninum determined by microsatellite markers
作者： N. Salehi , B. Gottstein , H.R. Haddadzadeh
DOI: 10.1016/J.PARINT.2015.05.005
关键词:
摘要: Neospora caninum is one of the most significant parasitic organisms causing bovine abortion worldwide. Despite economic impact this infection, relatively little known about genetic diversity parasite. In study, using Nc5 and ITS1 nested PCR, N. has been detected in 12 brain samples aborted fetuses from 298 seropositive dairy cattle collected four different regions Tehran, Iran. These specimen (Nc-Iran) were genotyped multilocus 9 microsatellite markers previously described (MS4, MS5, MS6A, MS6B, MS7, MS8, MS10, MS12 MS21). Microsatellite amplification was completely feasible 2 samples, semi-completely 8 failed samples. Within two performed allelic profiles Nc-Iran strains, unique obtained for both novel patterns found MS8 MS10 markers. The Jaccard's similarity index showed difference between these strains other standard isolates derived GenBank such as Nc-Liv, Nc-SweB1, Nc-GER1, KBA1, KBA2. All originating same area identical numbers a correlation number repeats geographic districts observed.
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