MicroRNA-30a downregulation contributes to chemoresistance of osteosarcoma cells through activating Beclin-1-mediated autophagy.

摘要: Abstract Autophagy has been recognized as an important element of tumor cell migration, invasion, and chemo-resistance, our previous results showed that Beclin-1-mediated autophagy contributed to osteosarcoma chemoresistance. However, the regulating mechanism is still unclear. In this study, aim was clarify microRNA (miRNA)-related mechanisms underlying followed by chemotherapy in osteosarcoma. First, miRNA screening using qRT-PCR identified miR-30a significantly reduced Dox-resistant cells. Second, activity increased while expression after agents indicated enhanced Beclin-1, conversion microtubule-associated protein LC3-I LC3-II. Furthermore, overexpression promoted chemotherapy-induced apoptosis responding chemotherapy. Moreover, rapamycin, promoter able partly reverse effect Luciferase reporter assay directly binds 3'-UTR Beclin-1 gene, which further confirmed chemoresistance via suppressing autophagy. Collectively these indicate its downstream target gene can be used treatment chemo-resistance future.