Detection of antibody to epizootic diarrhea of infant mice (EDIM) virus.

作者: Sheridan Jf , Smith Al , Knudson Dl , Paturzo Fx

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摘要: Cultured mouse liver cells containing either eipzootic diarrhea of infant mice viral antigen or Nebraska calf vaccine virus were equally effective for detecting serum antibody to epizootic by immunofluorescence. The presence rotavirus in intestinal suspensions used as cell culture inocula was confirmed polyacrylamide gel electrophoresis the double-stranded RNA genome. When immunofluorescence assay tested a limited comparison with an enzyme-linked immunosorbent using simian SA 11 antigen, fluorescence test specific but less sensitive than assay.

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