Lentiviral Vector Mediated Claudin1 Silencing Inhibits Epithelial to Mesenchymal Transition in Breast Cancer Cells
作者： Xianqi Zhao , Yanan Zou , Qingqing Gu , Guannan Zhao , Horace Gray
DOI: 10.3390/V7062755
关键词:
摘要: Breast cancer has a high incidence and mortality rate worldwide. Several viral vectors including lentiviral, adenoviral adeno-associated have been used in gene therapy for various forms of human cancer, shown promising effects controlling tumor development. Claudin1 (CLDN1) is member the tetraspan transmembrane protein family that plays major role tight junctions associated with metastasis. However, CLDN1 breast largely unexplored. In this study, we tested therapeutic potential silencing expression two (MDA-MB-231 MCF7) cell lines using lentiviral vector mediated RNA interference. We found short hairpin (shRNA) construct efficiently silenced both lines, knockdown resulted reduced proliferation, survival, migration invasion. Furthermore, inhibited epithelial to mesenchymal transition (EMT) by upregulating marker, E-cadherin, downregulating markers, smooth muscle alpha-actin (SMA) Snai2. Our data demonstrated interference great inhibiting EMT growth.
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