Granulocyte colony-stimulating factor stabilizes cardiac electrophysiology and decreases infarct size during cardiac ischaemic/reperfusion in swine.

摘要: Aim:  Effects of granulocyte colony-stimulating factor (G-CSF) on cardiac electrophysiology during ischaemic/reperfusion (I/R) period are unclear. We hypothesized that G-CSF stabilizes I/R injury by prolonging the effective refractory (ERP), increasing ventricular fibrillation threshold (VFT) and decreasing defibrillation (DFT), cardioprotection is via preventing mitochondrial dysfunction. Methods:  In intact-heart protocol, pigs were infused with either or vehicle (n = 7 each group) without induction. (0.33 μg kg−1 min−1) (n = 8 for 30 min prior to a 45-min left anterior descending artery occlusion at reperfusion. Diastolic pacing (DPT), ERP, VFT DFT determined in all before period. Rat’s isolated mitochondria used test protective effect (100 nm) H2O2-induced oxidative damage. Results:  Neither nor altered any parameter pigs. During ischaemic period, significantly increased DPT, ERP altering DFT. reperfusion, continued increase DPT other parameters. The infarct size was decreased group, compared vehicle. could also prevent swelling, decrease ROS production, membrane depolarization. Conclusion:  increases reduces size, thus stabilizing myocardial electrophysiology, fatal arrhythmia I/R. mechanism be its dysfunction.