Lack of Alpha-Synuclein Modulates Microglial Phenotype In Vitro

摘要: Alpha (α)-synuclein neuronal effects are continually being defined although its role in regulating glial phenotypes remains unclear. An ability to regulate microglial activation was investigated using primary cultures from wild type and α-synuclein deficient mice (Snca-/-). Snca-/- microglia demonstrated increased secretion of the cytokine tumor necrosis factor-alpha (TNF-α), impaired phagocytic ability, elevated prostaglandin levels, protein levels key enzymes lipid-mediated signaling events, cytosolic phospholipase (cPLA(2)), cyclooxygenase-2 (Cox-2) D2 (PLD2) when compared cells. Increased cPLA(2) Cox-2 were partially attenuated by inhibiting PLD-dependent with n-butanol treatment.