A high-throughput SNuPE assay for genotyping SNPs in the flanking regions of Zea mays sequence tagged simple sequence repeats

摘要: Information on genetic relationships among individuals is of importance to maize breeders for line and hybrid development. Estimates the similarity breeding materials best obtained using DNA markers. Single Nucleotide Polymorphisms small insertions/deletions are both emerging as a new generation markers, due their abundance amenability fully automated genotyping. Application SNPs, example in mapping projects or programs, involves analysis large number samples, therefore requires rapid, inexpensive, highly methods genotype sequence variants. Towards this, we have applied high throughput Primer Extension assay assess 23 polymorphic base variations at five microsatellite loci 22 inbred lines, well population two lines. Using MegaBACE genotyper, able more than 1248 (96 x 13) samples single 75 minute run. The SNuPE method successfully genotyped pair interest all It was also found that primers containing polymorphisms themselves could be used samples. This technique allows rapid production valuable information varieties. We further demonstrate utility this method, it map one loci.