Characterization of cellulose degradation during accelerated ageing by SEC-MALS, SEC-DAD, and A4F-MALS methods

摘要: Size exclusion chromatography (SEC) is currently the method most frequently used for molar mass determination of cellulose, being applied to either derivatized cellulose in form tricarbanilates (CTC) or nonderivatized solution lithium chloride dimethylacetamide 1,3-dimethyl-2-imidazolidinone. In this work, we compared three methods analysis paper subjected accelerated ageing: size combination with multiangle light scattering (SEC-MALS) detection, diode-array detector and calibration using polystyrene standards (SEC-DAD), asymmetric flow field–flow fractionation with multiangle (A4F-MALS). Prior separation, samples were phenyl isocyanate CTC dissolved in tetrahydrofuran. Each provided different absolute values mass, but pairwise correlations between them linear high correlation coefficients (r = 0.990–0.992). The highest obtained by A4F-MALS method; SEC-MALS SEC-DAD lower values, especially due shear degradation high-molecular chains.