Chemical and microbiological characterization of tinctures and microcapsules loaded with Brazilian red propolis extract

摘要: Abstract The aim of this study was to characterize tinctures and microcapsules loaded with an ethanol extract red propolis through chemical, physicochemical microbiological assays in order establish quality control tools for nutraceutical preparations propolis. markers (isoflavonoids, chalcones, pterocarpans, flavones, phenolic acids, terpenes guttiferones) present the tinctures A B were identified confirmed using LC/ESI/FTMS/Orbitrap. Four compositions (A, B, C D) prepared contain tincture the some pharmaceutical excipients submitted two drying processes, i. e. spray-drying freeze-drying obtain extract. total flavonoid content antioxidant activity tests. antibacterial minimum inhibitory concentration (MIC) tested Staphylococcus aureus ATCC 25293 Pseudomonas aeruginosa 27853 strains. presented high quantities from 20.50 40.79 mg/100 mg microcapsules. IC50 determined (IC50: 6.95 µg/mL 7.48 µg/mL), spray-dried 8.89–15.63 µg/mL) freeze-dried 11.83–23.36 µg/mL). proved be bioactive against gram-positive gram-negative bacteria inhibition halos superior 10 mm at 200 µg/mL MIC values 135.87–271.74 µg/mL strain 271.74–543.48 µg/mL strain. have a potential application products.