Genetic Redundancy in Iron and Manganese Transport in the Metabolically Versatile Bacterium Rhodopseudomonas palustris TIE-1.

摘要: ABSTRACT The purple nonsulfur bacterium Rhodopseudomonas palustris TIE-1 can produce useful biochemicals such as bioplastics and biobutanol. Production of requires intracellular electron availability, which is governed by the availability transport essential metals iron (Fe). Because distinct chemical properties ferrous [Fe(II)] ferric [Fe(III)], different systems are required for their storage in bacteria. Although Fe(III) well characterized, we know much less about Fe(II) except FeoAB system. Iron transporters also import manganese (Mn). We studied Fe Mn five putative under metal-replete, metal-depleted, oxic, anoxic conditions. observed that overexpressing feoAB, efeU, nramp1AB, concentrations be enhanced oxic conditions, respectively. deletion a single gene/operon does not attenuate or uptake regardless growth conditions used. This indicates genetically dissimilar yet functionally redundant complement each other. Relative gene expression analysis shows feoAB efeU expressed during depletion both promoters these transporter genes contain combination Fur Fnr boxes, suggesting regulated oxygen availability. findings from this study will help us modulate concentrations, ultimately improving TIE-1’s ability to desirable biomolecules. IMPORTANCERhodopseudomonas metabolically versatile use various donors, including poised electrodes, photoautotrophic growth. biomolecules, biofuels bioplastics, reduced biomolecules controlled which, turn, mediated iron-containing proteins cell. Several exist genome. Some Mn, part several important cellular enzymes. Therefore, understanding respond levels improve Our data suggest via plasmid-based expression, increase TIE-1. Future work leverage an attractive microbial chassis future biotechnological workhorse.