Mechanical stress modulates expression of toll-like receptors in human PDL cells

摘要: MECHANICAL STRESS MODULATES EXPRESSION OF TOLL-LIKE RECEPTORS IN HUMAN PDL CELLS Yaroslav R Yarmolyuk, DDS Marquette University, 2012 Orthodontic movement of teeth with compromised periodontium is risky because heavy orthodontic force could exacerbate the damaged periodontal status. Guidelines for moving periodontally-compromised have not been established due to lack scientific evidence about relationship between inflammation and mechanical force. In periodontitis, lipopolysaccharide (LPS) recognized in ligament (PDL) by tolllike receptors (TLR) which lead destruction tissues through inflammatory cascades. Recent studies demonstrated that absence TLR2 TLR4 leads reduced alveolar bone loss mice. Therefore, reduction on PDLF cells can a protective effect against attack pathogens and, accordingly, decrease susceptibility disease. The objective study was explore stress expression toll-like human fibroblasts (hPDLF).Human (hPDLF) were cultured glass slides. Upon confluence, starved 24 hours then subjected fluid shear (FSS) 12 dynes/cm 1 hour. After FSS, lysed test phosphorylation extracellular regulated kinase (ERK)1/2 expressions TLR4. To possible involvement ERK1/2 signaling pathway, specific inhibitor PD98059 added during flow. whole cell lysates from each group immunoblotted anti-TLR2 anti-TLR4 antibodies. signals interest determined using ECL method. For quantification, densitometries gel bands normalized vinculin. One-way ANOVA Tukey’s post hoc used compare results among experimental groups, p value being set at 0.05. As found, but abundantly expressed hPDLF cells. Compared static controls, FSS significantly When added, FSS-induced recovered back control level. Conclusively, downregulates TLR4, mediated MAPK (ERK1/2) pathway. Our findings suggest (mimicking light application) possibly alleviate status via down-regulation