Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides.
作者： Y Yamaji , T Kubota , K Sasaguri , S Sato , Y Suzuki
DOI: 10.1128/IAI.63.9.3576-3581.1995
关键词: TGF alpha 、 Gene expression 、 Lipopolysaccharide 、 Porphyromonas gingivalis 、 Biology 、 Transforming growth factor beta 、 Microbiology 、 Cytokine 、 Proinflammatory cytokine 、 Tumor necrosis factor alpha
摘要: The effects of Porphyromonas gingivalis lipopolysaccharide (P-LPS) and Escherichia coli (E-LPS) on the gene expression production inflammatory cytokines human periodontal ligament fibroblasts (HPLF) were examined by a Northern (RNA blot) assay enzyme-linked immunosorbent assay, respectively. mRNAs for interleukin-6 (IL-6), IL-8, transforming growth factor beta (TGF-beta) detected in HPLF cells, but IL-1 alpha, beta, tumor necrosis granulocyte-macrophage colony-stimulating not reverse transcription-PCR. TGF-beta mRNA was influenced either LPS. P-LPS (1 to 10 micrograms/ml) E-LPS (100 markedly stimulated IL-6 IL-8 compared with control. synthesis also 100 micrograms both LPSs per ml, at 1 microgram/ml. Secretion into culture medium 6 3 h, respectively, after exposure (10 micrograms/ml). These findings suggested that P. leads tissue destruction alveolar bone resorption through released from cells its
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