Adjacent insertion sequences IS2 and IS5 in bacteriophage Mu mutants and an IS5 in a lambda darg bacteriophage.

摘要: Using electron microscopic heteroduplex analysis, we have demonstrated that an insertion found in a Mu prophage and some infectious. deletion-substitution mutants derived from it consists of bacterial sequence IS2 linked directly to IS5. Other infectious the same lysogen only IS5 or portion IS2. In addition, independent transducing phage, lambda 13 dargB2, is The ends are short, inverted duplications each other. These observations support notion DNA previously designated on basis single example KH100 bona fide sequence.