Establishment in monolayer culture and characterization of four human melanoma cell lines

摘要: Four new human melanoma cell lines were established in monolayer culture from xenograft originating different patients. Several distinct characteristics of the source retained lines, e.g., number chromosomes, DNA-index, and ultrastructure. Cell volume was generally larger for than corresponding but differences among similar vitro vivo. The showed significant growth pattern, i.e., motility degree intercellular contact. cycle time (Tc) during exponential ranged 15 to 21 h. Tc mainly due duration S. Growth fraction close 100% loss negligible growth. Plating efficiency 90–100% presence feeder cells. four represent a valuable supplement future studies biology, pathophysiology, metastatic behavior, treatment sensitivity malignant melanoma.