Decreases in circulating CD4+CD25hiFOXP3+ cells and increases in intragraft FOXP3+ cells accompany allograft rejection in pediatric liver allograft recipients.

摘要: We examined CD4(+)CD25(hi)FOXP3(+) cells Treg in children following liver transplantation and determined the relationship between cell levels blood graft. Peripheral was obtained from pediatric transplant patients at sequential time points: pre-transplant, one month, 3-4 months, 6-7 11-12 months post-transplant. PBMC were isolated, labeled for CD4, CD25 FOXP3 expression analyzed by flow cytometry cells. Sorted CD4(+)CD25(hi) assessed functional activity. Pretransplant of not significantly different post-transplant However, decreased during acute rejection compared with when graft function stable. Immunohistochemistry revealed that FOXP3(+) increased portal region livers histopathologic evidence without localized primarily within inflammatory infiltrate. These data indicate are found site allograft may play a role regulation alloreactivity. Moreover, monitoring peripheral be useful improving management recipients.