Quantifying thrombin-catalyzed release of fibrinopeptides from fibrinogen using high-performance liquid chromatography.
作者： Assunta S. Ng , Sidney D. Lewis , Jules A. Shafer
DOI: 10.1016/0076-6879(93)22023-9
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摘要: Publisher Summary This chapter focuses on the quantifying thrombin-catalyzed release of fibrinopeptides from fibrinogen using high-performance liquid chromatography (HPLC). It presents several methods in which HPLC analysis can yield (1) initial rates fibrinogen, (2) Miehaelis–Menten parameters catalytic constant( k CAT ) and (Michaelis constant) K M for a-thrombin-catalyzed fibrinopeptide A (FPA) fibilnopeptide B (FPB) fibrin I, (3) specificity constant / both FPA FPB at concentrations below , (4) inhibition hirugen, an exosite inhibitor α-thrombin. The also discusses profile coeluting activation peptides factor XIII. describes a method determining concentration α-thrombin that is catalytically competent to process fibrinogen.
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sci-hub.se 参考文章(44)
OC Leeksma, F Meijer-Huizinga, EA Stoepman-van Dalen, CJ van Ginkel, WG van Aken, JA van Mourik, Fibrinopeptide A and the Phosphate Content of Fibrinogen in Venous Thromboembolism and Disseminated Intravascular Coagulation Blood. ,vol. 67, pp. 1460- 1467 ,(1986) , 10.1182/BLOOD.V67.5.1460.1460
S D Lewis, P P Shields, J A Shafer, Characterization of the kinetic pathway for liberation of fibrinopeptides during assembly of fibrin. Journal of Biological Chemistry. ,vol. 260, pp. 10192- 10199 ,(1985) , 10.1016/S0021-9258(17)39231-1
D L Higgins, S D Lewis, J A Shafer, Steady state kinetic parameters for the thrombin-catalyzed conversion of human fibrinogen to fibrin. Journal of Biological Chemistry. ,vol. 258, pp. 9276- 9282 ,(1983) , 10.1016/S0021-9258(17)44663-1
M.C. Naski, J.A. Shafer, A kinetic model for the alpha-thrombin-catalyzed conversion of plasma levels of fibrinogen to fibrin in the presence of antithrombin III. Journal of Biological Chemistry. ,vol. 266, pp. 13003- 13010 ,(1991) , 10.1016/S0021-9258(18)98795-8
M.C. Naski, J.W. Fenton, J.M. Maraganore, S.T. Olson, J.A. Shafer, The COOH-terminal domain of hirudin. An exosite-directed competitive inhibitor of the action of alpha-thrombin on fibrinogen. Journal of Biological Chemistry. ,vol. 265, pp. 13484- 13489 ,(1990) , 10.1016/S0021-9258(18)77372-9
Martin L. Schwartz, Salvatore V. Pizzo, Robert L. Hill, Patrick A. McKee, The Subunit Structures of Human Plasma and Platelet Factor XIII (Fibrinstabilizing Factor) Journal of Biological Chemistry. ,vol. 246, pp. 5851- 5854 ,(1971) , 10.1016/S0021-9258(18)61888-5
J W Fenton, M J Fasco, A B Stackrow, Human thrombins. Production, evaluation, and properties of alpha-thrombin. Journal of Biological Chemistry. ,vol. 252, pp. 3587- 3598 ,(1977) , 10.1016/S0021-9258(17)40293-6
D.L. Higgins, J.A. Shafer, Fibrinogen Petoskey, a dysfibrinogenemia characterized by replacement of Arg-A alpha 16 by a histidyl residue. Evidence for thrombin-catalyzed hydrolysis at a histidyl residue. Journal of Biological Chemistry. ,vol. 256, pp. 12013- 12017 ,(1981) , 10.1016/S0021-9258(18)43225-5
R.R. Hantgan, J. Hermans, Assembly of fibrin. A light scattering study. Journal of Biological Chemistry. ,vol. 254, pp. 11272- 11281 ,(1979) , 10.1016/S0021-9258(19)86481-5
M C Naski, J A Shafer, Alpha-thrombin-catalyzed hydrolysis of fibrin I. Alternative binding modes and the accessibility of the active site in fibrin I-bound alpha-thrombin. Journal of Biological Chemistry. ,vol. 265, pp. 1401- 1407 ,(1990) , 10.1016/S0021-9258(19)40028-8