PI3-Kinase Upregulation and Involvement in Spontaneous Tone in Arteries From DOCA-Salt Rats: Is p110δ the Culprit?

摘要: Increased expression of phosphoinositide 3-kinase (PI3-kinase) mediates elevated tone in the aorta from hypertensive deoxycorticosterone acetate (DOCA)-salt rats. In this article, we hypothesized that (1) alterations observed with respect to PI3-kinase would also occur mesenteric resistance arteries responsible for determining total peripheral (TPR) and (2) p110δ activity was increased localized vascular smooth muscle cells (VSMCs), increase spontaneous aortae DOCA-salt Mesenteric were isolated (190±3 mm Hg) sham (121±2 Myograph experiments revealed LY294002 (20 μmol/L), a inhibitor, significantly decreased rats as compared (−49±12 mg versus −10±7 mg). Western analyses artery protein homogenate p85α subunit protein, levels (0.30±0.07 0.16±0.04% alpha-actin arbitrary units). Immunohistochemistry p110δ-specific staining VSMCs, more intense Compared sham, p110δ-associated (158% sham) likely because specific inhibitor IC87114 concentration-dependent manner. Collectively, these data further implicate isoform arterial hyperresponsiveness hypertension at level both large small arteries.