Analysis of the thymidylate synthase gene structure in colorectal cancer patients and its possible relation with the 5-Fluorouracil drug response.
作者： A. Calascibetta , Flavia Contino , S. Feo , G. Gulotta , M. Cajozzo
DOI: 10.4061/2010/306754
关键词:
摘要: Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms TS in tumour cell lines confer resistance fluoropyrimidines. We planned perform whole gene structure by means sequencing techniques human colorectal cancer (CRC) samples try identify presence any possible form could be responsible fluoropyrimidines drug worse prognosis. performed TS-DNA sequence 68 CRC from patients A, B, C Dukes' stages different histological grade, but we did not find mutation structure. In future intend widen analysis metastatic CRCs, because due their higher genomic instability, they present a
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