The Endocrine Disruptor Mono-(2-Ethylhexyl) Phthalate Affects the Differentiation of Human Liposarcoma Cells (SW 872)

摘要: Esters of phthalic acid (phthalates) are largely used in industrial plastics, medical devices, and pharmaceutical formulations. They easily released from plastics into the environment can be found measurable levels human fluids. Phthalates agonists for peroxisome proliferator-activated receptors (PPARs), through which they regulate translocator protein (TSPO; 18 kDa) transcription a tissue-specific manner. TSPO is drug- cholesterol-binding involved mitochondrial respiration, steroid formation, cell proliferation. has been shown to increase during differentiation decrease maturation mouse adipocytes. The purpose this study was establish effect mono-(2-ethylhexyl) phthalate (MEHP) on SW 872 preadipocyte cells, examine role process. After 4 days treatment with 10 µM MEHP, we observed changes acetyl-CoA carboxylase alpha, adenosine triphosphate citrate lyase, glucose transporters 1 4, S100 calcium binding B, all markers differentiation. These gene expression coincided cellular proliferation without affecting triglyceride content. Taken together, these data suggest that MEHP exerts differentiating preadipocytes. Interestingly, able temporarily mRNA PPAR-α β/δ pathways. results considered an important player process itself, or alternatively factor whose presence essential adipocyte development.