Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells.

摘要: Abstract In this report, we demonstrate the sequence-specific suppression of viral expression in T cells chronically infected with human immunodeficiency virus 1 (HIV-1), using antisense phosphorothioate oligodeoxynucleotides. As a target for intervention, used HIV-1 gene rev, which is essential replication and regulates virion proteins, part, by affecting splicing mRNA. A oligomer complementary to initiation sequence rev had significant selective inhibitory effect on production several proteins HIV-1-infected drastically reduced unspliced (genomic) mRNA transcripts, relative sparing smaller (spliced) transcripts. By contrast, unmodified normal phosphodiester linkages as well oligomers containing sense, random, homopolymeric sequences, or N3-methylthymidine residues did not have an expression. Thus, specificity nuclease resistance were critical anti-viral-gene regulatory molecules. The altered profile induced suggests that mechanism inhibition due interference gene, translation arrest.