mRNA and miRNA Expression Analyses of the MYC/E2F/miR-17-92 Network in the Most Common Pediatric Brain Tumors.

摘要: Numerous molecular factors disrupt the correctness of cell cycle process leading to development cancer due increased proliferation. Among known causative such is abnormal gene expression. Nowadays in light current knowledge alterations are frequently considered context mRNA-miRNA correlation. One with potential value tumorigenesis feedback loop between MYC and E2F genes which miR-17-5p miR-20a from miR-17-92 cluster involved. The literature shows that overexpression members OncomiR-1 involved many solid tumors. In present work, we investigated expression components MYC/E2F/miR-17-92 network their closely related elements including families miRNAs two paralogs miR-17-92: miR-106b-25 miR-106a-363, most common brain tumors childhood, pilocytic astrocytoma (PA), WHO grade 1; ependymoma (EP), 2; medulloblastoma (MB), 4. We showed highest was observed family for MYCN E2F2. Positive correlation tumor type, being noted medulloblastomas, followed by ependymomas, lowest astrocytomas. Most miR-17-92, miR-106a-363 clusters were upregulated miR-18a miR-18b. rest miRNAs, miR-19a, miR-92a, miR-106a, miR-93, or miR-25 also high values. miR-17-5p, obtained a level medulloblastomas while close control samples. miRNA depended on histology.