An ankyrin-like protein with transmembrane domains is specifically lost after oncogenic transformation of human fibroblasts.

摘要: We have identified a novel transformation-sensitive mRNA, which is present in cultured fibroblasts but lacking SV40 transformed cells as well many mesenchymal tumor cell lines. The corresponding gene located on human chromosome 8 band 8q13. open reading frame of the mRNA encodes protein 1119 amino acids forming two distinct domains. N-terminal domain consists 18 repeats that are related to cytoskeletal ankyrin. C-terminal contains six putative transmembrane segments resemble ion channels. This overall structure reminiscent TRP-like proteins function store-operated calcium with anMr 130 kDa expressed at very low level and moderate liposarcoma cells. Overexpression eukaryotic appears interfere normal growth, suggesting it might play direct or indirect role signal transduction growth control.