Sequencing of plasmids pAMBL1 and pAMBL2 from Pseudomonas aeruginosa reveals a blaVIM-1 amplification causing high-level carbapenem resistance.

摘要: BACKGROUND: Carbapenemases are a major concern for the treatment of infectious diseases caused by Gram-negative bacteria. Although plasmids responsible spread resistance genes among these pathogens, there is limited information on nature mobile genetic elements carrying carbapenemases in Pseudomonas aeruginosa. METHODS: We combined data from two different next-generation sequencing platforms, Illumina HiSeq2000 and PacBio RSII, to obtain complete nucleotide sequences blaVIM-1-carrying (pAMBL1 pAMBL2) isolated P. aeruginosa clinical isolates. RESULTS: Plasmid pAMBL1 has 26 440 bp carries RepA_C family replication protein. similar pNOR-2000 pKLC102 pAX22 Achromobacter xylosoxidans, which also carry VIM-type carbapenemases. pAMBL2 24 133 plasmid with protein that belongs Rep_3 family. It shows high degree homology fragment blaVIM-1-bearing pPC9 putida. three copies blaVIM-1 cassette an In70 class 1 integron conferring, unlike pAMBL1, high-level carbapenems. CONCLUSIONS: present new coding VIM-1 carbapenemase report presence produces carbapenems.