Loss‐of‐function of OsSTN8 suppresses the photosystem II core protein phosphorylation and interferes with the photosystem II repair mechanism in rice (Oryza sativa)

摘要: SUMMARYSTN8 kinase is involved in photosystem II (PSII) core protein phosphorylation (PCPP). To examine the roleof PCPP PSII repair during high light (HL) illumination, we characterized a T–DNA insertional knockoutmutant of rice (Oryza sativa) STN8 gene. In this osstn8 mutant, was significantly suppressed, andthe grana were thin and elongated. Upon HL strongly inactivated mutants, butthe D1 degraded more slowly than wild-type, mobilization supercomplexesfrom to stromal lamellae for also suppressed. addition, higher accumulation ofreactive oxygen species preferential oxidation reaction center proteins thylakoid mem-branes observed mutants illumination. Taken together, our current data show thatthe absence sufficient abolish produce all phenotypesobserved double mutant Arabidopsis, indicating essential role STN8-mediated PSIIrepair.Keywords: kinase, phosphorylation, proteindegradation, repair, rice.INTRODUCTIONDuring photosynthesis, plants convert into chemicalenergy, but excessive harmful plants. Even inlow light, may be damaged pro-portion intensity (Tyystj€arvi Aro, 1996; Jan-sen et al., 1999; Oguchi 2009). A mustbe repaired at intensities, overall level ofphotosynthetic activity reduced when rate repairdoes not match photodamage (Prasil al.,1992). The photodamaged PSII, particularly turn-over protein, thought key regulatorystep cycle (Yokthongwattana Melis,2006). cycle, phosphorylated PSIIis first relocated from lamellae,where after dephos-phorylation by specific proteases (Bailey 2002; Kapri-Pardes 2007; Sun 2010; Kley 2011;Schuhmann Adamska, 2012; Wagner Kir-chhoff, 2013a), newly synthesized inserted intoPSII (Ohad 1985; Aro 1993; Rintam€aki al.,1996; Tikkanen 2008; 2012).The phosphorylation/dephosphorylation PSIIcore has been identified as one regulatorymechanisms that responsible protecting fromphotoinhibition (Tikkanen Fristedt 2009;