Redox, haem and CO in enzymatic catalysis and regulation

摘要: The present paper describes general principles of redox catalysis and regulation in two diverse systems. first is microbial metabolism CO by the Wood–Ljungdahl pathway, which involves conversion or H 2 /CO into acetyl-CoA, then serves as a source ATP cell carbon. focus on enzymes that make utilize CO, CODH (carbon monoxide dehydrogenase) ACS (acetyl-CoA synthase). In this converts generates acetyl-CoA reaction involving Ni·CO, methyl-Ni acetyl-Ni catalytic intermediates. A 70 (1 A=0.1 nm) channel guides generated at active site CODH, to ‘cage’ near sequester reactive species assure its rapid availability participate kinetically coupled with an unstable Ni(I) state was recently trapped photolytic, kinetic spectroscopic studies. also studies haem-regulated systems involve principle metabolic interlinking redox, haem CO. Recent HO2 (haem oxygenase-2), K + ion (the BK channel) nuclear receptor (Rev-Erb) demonstrate mode thiol–disulfide switch regulates binding gas signalling molecules (CO NO) modulate effect haem.