Immunological responses to injury and grafting in the central nervous system of nonhuman primates.

摘要: Abstract Allogeneic transplantation for the therapy of human Parkinson’s disease is being considered as a viable approach at several clinical centers worldwide. As an attempt to understand basic biology central nervous system (CNS) transplantation, our laboratory has developed experimental nonhuman primate model and carried out preliminary studies directed evaluating potential pathology graft site. In addition, have been conducted examine whether such procedures lead specific and/or nonspecific immunologic sensitization host or results in generalized immunosuppression. Groups rhesus macaques ( Macaca mulatta ) were either controls operated n = 6), autografted with adrenal medullary peripheral nerve tissue 3), allografted fetal mesencephalic 6). Immunohistological demonstrated presence mononuclear cell infiltrates early 1 wk up yr postoperatively, although frequency infiltrating cells declined time. The consisted variable numbers which express CD2+, CD3+, CD4+, CD8+, CD19+, CD22+, CD25+, CD68+. There appeared be no difference frequency, kinetics, phenotype operative compared recipients auto- allografts. Tissue sections obtained postoperatively showed low levels major histocompatibility complex (MHC) Class I antigens detectable level MHC-Class II neural tissue. A small aliquot from site was placed vitro media containing interleukin-2 (IL-2), led exudation growth that predominantly CD4+ cells. Phenotypic blood (PBMC) controls, allograft recipient monkeys performed varying time periods failed show differences frequencies subsets T-cells, B-cells, NK-cells, monocytes. Studies on aliquots same PBMC functional LAK cells, response polyclonal mitogens. Finally, allogeneic grafts evidence donor-specific humoral cellular sensitization. These data indicate autograft tissues CNS did not induce nor