Quantification of TSPO overexpression in a rat model of local neuroinflammation induced by intracerebral injection of LPS by the use of [(18)F]DPA-714 PET.

摘要: [18F]DPA-714 is a radiotracer with high affinity for TSPO. We have characterized the kinetics of in rat brain and evaluated its ability to quantify TSPO expression PET using neuroinflammation model induced by unilateral intracerebral injection lipopolysaccharide (LPS). Dynamic small-animal scans were performed Wistar rats on FOCUS-220 system up 3 h. Both plasma perfused homogenates analysed HPLC radiometabolites. Full kinetic modelling uptake was metabolite-corrected arterial input function. Binding potential (BPND) calculated as distribution volume ratio minus one (DVR−1) between affected healthy tissue used outcome measure against reference models. The percentage intact samples 92 ± 4 % at 10 min, 75 ± 8 % 40 min 52 ± 6 % 180 min. radiometabolite fraction negligible (<3 % 30 min). Among models investigated, reversible two-tissue (2T) compartment best described kinetics. BPND values obtained simplified multilinear (SRTM, MRTM) contralateral striatum region correlated well (Spearman’s r = 0.96, p ≤ 0.003) 2T DVR−1, showed comparable bias (bias range 17.94 %, 20.32 %). Analysis stability over time suggested that acquisition should be least 90 min SRTM MRTM. Quantification binding full feasible model. MRTM can reasonable substitutes scan duration 90 min. However, selection depends disease used.