Liquid chromatographic determination of geometrical retinol isomers and carotene in enteral feeding formulas.

摘要: The objective of the present study was to evaluate a practical method for separation and determination geometrical retinol isomers carotene in enteral feeding formulas analyze 17 samples commercial formulas. By using normal-phase HPLC column mobile phase consisting 1-octanol n-hexane, seven were separated identified from standard solution after photolysis. For evaluation Vitamin A activity these formulas, simultaneous total performed. data about linearity, recovery, accuracy precision showed reliability analytical procedures. In unsaponifiable portion six identified: (E)-retinol; (Z)-13; (Z)-9; (Z,Z)-9,13; (Z,Z)-11,13 (Z)-7-retinol. (Z)-13/(E)-retinol ratios ranged between 3 37%. range Z/(E)-retinol fell 5 42%. Despite high concentration Z-isomers observed various none presented below 90% that specified on label.