Reversed-phase high-performance liquid chromatographic separation and electrochemical detection of retinol and its isomers
作者： W.A. MacCrehan , Schönberger
DOI: 10.1016/0378-4347(87)80092-0
关键词:
摘要: Abstract Baseline separation of the isomers retinol using reversed-phase high-performance liquid chromatography (HPLC) in less than 30 min is presented. A new approach to detection electrochemical developed. The oxidative electrochemistry studied at a glassy-carbon electrode coulometry, ultraviolet-visible spectrophotometry and HPLC. Amperometric HPLC for provided linear response from 0 1.5 μg/ml limit 4.1 ng/ml. Electrochemical was compared absorbance determination human serum extracts. Good agreement found results obtained with two detectors.
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