HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF RETINOID ISOMERS : AN OVERVIEW

摘要: Publisher Summary The separation and quantitation of different retinoid isomers is essential in studies retinoids the visual systems vertebrates invertebrates, investigations isomer interconversion other tissues, determining biopotency vitamin A foodstuffs food supplements such as fish liver oils. various mono-cis comparatively easy provided only a single class derivatives under study. However, several classes widely differing polarities are present mixture being investigated. It usually to develop highly efficient method separating particular retinoids. For most classes, with exception retinoic acid, normal-phase have been used almost exclusively. Normal-phase also useful chapter shows an example variety ranging polarity from 11-cis-retinyl palmitate all-trans-3-dehydroretinol. summarizes for isomers. major component mobile phase nearly always n-hexane, although solvents sporadically used.