Chronic hypoxia potentiates age-related oxidative imbalance in brain vessels and synaptosomes.
作者： Cristina Carvalho , Maria S. Santos , Ines Baldeiras , Catarina R. Oliveira , Raquel Seica
DOI: 10.2174/156720210793180765
关键词:
摘要: This study was aimed to evaluate and compare the effect of chronic hypoxia aging in oxidative status brain vessels synaptosomes. For this purpose we isolated synaptosomes from 3- 12-month-old rats subjected (10% O2 for 7 days) or normoxia (21% O2). Several parameters were evaluated: mitochondrial aconitase activity, hydrogen peroxide (H2O2) malondialdehyde (MDA) levels enzymatic [superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) reductase (GR)] non-enzymatic [glutathione (GSH), disulfide (GSSG) vitamin E] antioxidant defences. Concerning vessels, observed an age-dependent increase MDA SOD, GR GPx activities. In young animals, induced H2O2, GSSG E CuZnSOD catalase activities a decrease GSH levels. mature GSH/GSSG ratio, aconitase, MnSOD H2O2 levels, activity. activity Our results show that promotes potentiates imbalance predisposing neurodegeneration. Further, are differently affected by supporting idea existence tissue-specific susceptibilities.
eurekaselect.com参考文章(97)
Bàrbara Segura i Fàbregas, Ma Ángeles María Ángeles Jurado, None, Síndrome metabólico y envejecimiento: déficit cognitivo y alteraciones estructurales del sistema nervioso central Revista De Neurologia. ,vol. 49, pp. 417- 424 ,(2009) , 10.33588/RN.4908.2008425
Xi-long Zhang, Jian-nan Liu, Gan Lu, Guo-yong Yin, Di Yang, Jie-xin Zhang, Yan Qiu, The effect of oxidative stress in myocardial cell injury in mice exposed to chronic intermittent hypoxia. Chinese Medical Journal. ,vol. 123, pp. 74- 78 ,(2010)
L. Flohé, F. ötting, Superoxide dismutase assays. Methods in Enzymology. ,vol. 105, pp. 93- 104 ,(1984) , 10.1016/S0076-6879(84)05013-8
Ronald W. Estabrook, [7] Mitochondrial respiratory control and the polarographic measurement of ADP : O ratios Methods in Enzymology. ,vol. 10, pp. 41- 47 ,(1967) , 10.1016/0076-6879(67)10010-4
P. Venditti, T. De Leo, S. Di Meo, Determination of tissue susceptibility to oxidative stress by enhanced luminescence technique. Methods in Enzymology. ,vol. 300, pp. 245- 252 ,(1999) , 10.1016/S0076-6879(99)00131-7
Allan G. Gornall, Charles J. Bardawill, Maxima M. David, Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry. ,vol. 177, pp. 751- 766 ,(1949) , 10.1016/S0021-9258(18)57021-6
Didem Sener Dede, Bunyamin Yavuz, Burcu Balam Yavuz, Mustafa Cankurtaran, Meltem Halil, Zekeriya Ulger, Eylem Sahin Cankurtaran, Kudret Aytemir, Giray Kabakci, Servet Ariogul, Assessment of Endothelial Function in Alzheimer's Disease: Is Alzheimer's Disease a Vascular Disease? Journal of the American Geriatrics Society. ,vol. 55, pp. 1613- 1617 ,(2007) , 10.1111/J.1532-5415.2007.01378.X
Leopold Flohé, Wolfgang A. Günzler, ASSAYS OF GLUTATHIONE PEROXIDASE Methods in Enzymology. ,vol. 105, pp. 114- 121 ,(1984) , 10.1016/S0076-6879(84)05015-1
D.H. Flint, J.F. Tuminello, M.H. Emptage, The inactivation of Fe-S cluster containing hydro-lyases by superoxide. Journal of Biological Chemistry. ,vol. 268, pp. 22369- 22376 ,(1993) , 10.1016/S0021-9258(18)41538-4
Gustavo Barja, Mitochondrial oxygen radical generation and leak: sites of production in states 4 and 3, organ specificity, and relation to aging and longevity. Journal of Bioenergetics and Biomembranes. ,vol. 31, pp. 347- 366 ,(1999) , 10.1023/A:1005427919188