In situ development of self-defensive antibacterial biomaterials: phenol-g-keratin-EC based bio-composites with characteristics for biomedical applications

摘要: Recently, the development of highly inspired biomaterials with multi-functional characteristics has gained considerable attention, especially in biomedical and other health-related areas modern world. It is well known that lack antibacterial potential significantly limited for many challenging applications such as infection-free wound healing and/or tissue engineering. In this perspective, a series novel bio-composites natural phenols functional entities keratin-EC base material were synthesised by laccase-assisted grafting. Subsequently, resulting composites removed from their respective casting surfaces critically evaluated biocompatibility features. Information also given on degradation profile soil. situ phenol-g-keratin-EC possess strong anti-bacterial activity against Gram-positive Gram-negative bacterial strains, i.e., B. subtilis NCTC 3610, S. aureus 6571, E. coli NTCT 10418 P. aeruginosa 10662. More specifically, 10HBA-g-keratin-EC 20T-g-keratin-EC 100% resistant to colonisation all aforementioned whereas 15CA-g-keratin-EC 15GA-g-keratin-EC showed almost negligible colonisation. Moreover, at various phenolic concentrations, it was clear newly remained cytocompatible human keratinocyte-like HaCaT, an obvious cell ingrowth tendency observed during neutral red dye uptake assay. From point view, increase rate recorded soil burial analyses. Our investigations could encourage greater utilisation materials sophisticated applications.