In situ proximity ligation assay for microscopy and flow cytometry.
作者： Karl‐Johan Leuchowius , Irene Weibrecht , Ola Söderberg
DOI: 10.1002/0471142956.CY0936S56
关键词:
摘要: The ability to study proteins and protein interactions inside cells tissues is important for elucidating how function in health disease. situ proximity ligation assay (in PLA) presented here can be used visualize proteins, protein-protein interactions, post-translational modifications tissues. method based upon the use of antibodies that target involved an interaction; hence, has advantage it clinical specimens, providing localized, quantifiable single molecule detection cells. This unit describes PLA with fluorescence microscopy flow cytometry (obtaining high sensitivity specificity detection) interactions. It also includes information on expected results troubleshoot assay.
sci-hub.se 参考文章(13)
Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola, Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation Molecular Cell. ,vol. 9, pp. 789- 798 ,(2002) , 10.1016/S1097-2765(02)00496-3
B. Sehat, A. Tofigh, Y. Lin, E. Trocme, U. Liljedahl, J. Lagergren, O. Larsson, SUMOylation mediates the nuclear translocation and signaling of the IGF-1 receptor. Science Signaling. ,vol. 3, ,(2010) , 10.1126/SCISIGNAL.2000628
Ola Söderberg, Mats Gullberg, Malin Jarvius, Karin Ridderstråle, Karl-Johan Leuchowius, Jonas Jarvius, Kenneth Wester, Per Hydbring, Fuad Bahram, Lars-Gunnar Larsson, Ulf Landegren, Direct Observation of Individual Endogenous Protein Complexes in Situ by Proximity Ligation Nature Methods. ,vol. 3, pp. 995- 1000 ,(2006) , 10.1038/NMETH947
Anne K. Kenworthy, Imaging Protein-Protein Interactions Using Fluorescence Resonance Energy Transfer Microscopy Methods. ,vol. 24, pp. 289- 296 ,(2001) , 10.1006/METH.2001.1189
Elena I. Frolova, Rodion Gorchakov, Larisa Pereboeva, Svetlana Atasheva, Ilya Frolov, Functional Sindbis virus replicative complexes are formed at the plasma membrane. Journal of Virology. ,vol. 84, pp. 11679- 11695 ,(2010) , 10.1128/JVI.01441-10
Karl-Johan Leuchowius, Malin Jarvius, Malin Wickström, Linda Rickardson, Ulf Landegren, Rolf Larsson, Ola Söderberg, Mårten Fryknäs, Jonas Jarvius, High Content Screening for Inhibitors of Protein Interactions and Post-translational Modifications in Primary Cells by Proximity Ligation Molecular & Cellular Proteomics. ,vol. 9, pp. 178- 183 ,(2010) , 10.1074/MCP.M900331-MCP200
Karl-Johan Leuchowius, Irene Weibrecht, Ulf Landegren, Lars Gedda, Ola Söderberg, Flow cytometric in situ proximity ligation analyses of protein interactions and post-translational modification of the epidermal growth factor receptor family Cytometry Part A. ,vol. 75, pp. 833- 839 ,(2009) , 10.1002/CYTO.A.20771
Helena Johansson, Frida Svensson, Rikard Runnberg, Tomas Simonsson, Stina Simonsson, Phosphorylated Nucleolin Interacts with Translationally Controlled Tumor Protein during Mitosis and with Oct4 during Interphase in ES Cells PLOS ONE. ,vol. 5, ,(2010) , 10.1371/JOURNAL.PONE.0013678
Malin Jarvius, Janna Paulsson, Irene Weibrecht, Karl-Johan Leuchowius, Ann-Catrin Andersson, Carolina Wählby, Mats Gullberg, Johan Botling, Tobias Sjöblom, Boyka Markova, Arne Östman, Ulf Landegren, Ola Söderberg, In SituDetection of Phosphorylated Platelet-derived Growth Factor Receptor β Using a Generalized Proximity Ligation Method Molecular & Cellular Proteomics. ,vol. 6, pp. 1500- 1509 ,(2007) , 10.1074/MCP.M700166-MCP200
Anne E Carpenter, Thouis R Jones, Michael R Lamprecht, Colin Clarke, In Kang, Ola Friman, David A Guertin, Joo Chang, Robert A Lindquist, Jason Moffat, Polina Golland, David M Sabatini, CellProfiler: image analysis software for identifying and quantifying cell phenotypes Genome Biology. ,vol. 7, pp. 1- 11 ,(2006) , 10.1186/GB-2006-7-10-R100