Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture

摘要: Efficient pre-replication complex (pre-RC) formation on chromatin templates is crucial for the maintenance of genome integrity. However, regulation dynamics during this process has remained elusive. We found that a conserved protein, GRWD1 (glutamate-rich WD40 repeat containing 1), binds to two representative replication origins specifically G1 phase in CDC6- and Cdt1-dependent manner, depletion reduces loading MCM but not CDC6 Cdt1. Furthermore, immunoprecipitation coupled with high-throughput sequencing (Seq) revealed significant genome-wide co-localization CDC6. histone-binding activity. To investigate effect architecture, we used formaldehyde-assisted isolation regulatory elements (FAIRE)-seq or FAIRE-quantitative PCR analyses, results suggest regulates openness at specific locations. Taken together, these findings may be novel protein origins.