Detection of nivalenol genotoxicity in cultured cells and multiple mouse organs by the alkaline single-cell gel electrophoresis assay

摘要: We tested the genotoxicity of nivalenol (NIV), a potent toxic trichothecene from Fusarium nivale, in cultured CHO cells and several mouse organs tissues (liver, kidney, thymus, bone marrow mucosa stomach, jejunum, colon) using alkaline single-cell gel electrophoresis (SCG, or Comet) assay. NIV at 50 100 micrograms/ml damaged nuclear DNA absence S9 mix, showing that was direct mutagen. In an vivo study, mice were sacrificed 2, 4, 8 h after either oral (20 mg/kg) intraperitoneal (3.7 administration NIV. damage measured by SCG assay as modified us. After dosing, appeared kidney 2 (returning to almost control level within following h), colon h, respectively. Liver thymus not damaged. injection, no any except for colon, where extensive observed, h. For histopathological examination, No necrotic changes detected yielded statistically significant damage. To measure effect on transport activity mice, 10 ml/kg (same volume treatments) 1% brilliant blue FCF (BB) administered orally. Thirty minutes later, BB reached simultaneous mg/kg, dissolved ml solution) did affect dye rate. Thus, strong yet delayed may follow systemic absorption rather than topical effect. As mutagen, showed organ specific time intensity.