Mycotoxin Nivalenol Induces Apoptosis and Intracellular Calcium Ion-Dependent Interleukin-8 Secretion but Does Not Exert Mutagenicity
作者： Hitoshi Nagashima , Hiroyuki Nakagawa , Keiko Iwashita
DOI: 10.1007/978-1-4020-9646-4_46
关键词:
摘要: To elucidate the mechanism underlying toxicity of nivalenol, we investigated cytotoxicity this mycotoxin to human promyelocyte-derived cell line HL60 after 24-h exposure. Internucleosomal DNA fragmentation, a hallmark apoptosis, was apparent at 3 and 10 µg/ml nivalenol. The fragmentation these concentrations proportional morphologic damage they induced in our previous studies, indicating that apoptosis led changes morphology. In addition,1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM), which chelates intracellular calcium ion, considerably attenuated nivalenol-induced interleukin (IL)-8 secretion from cells, thereby induction is ion-dependent. Further, even 50 µg/ml, nivalenol failed induce mutagenesis umu test regardless treatment with S9 fraction rat liver homogenate (which rich drug-metabolizing enzymes). Therefore, unlikely be mutagenic.
springer.com
sci-hub.st 参考文章(13)
Masahiro Matsubara, Tadafumi Tamura, Kenji Ohmori, Kazuhide Hasegawa, Histamine H1 receptor antagonist blocks histamine-induced proinflammatory cytokine production through inhibition of Ca2+-dependent protein kinase C, Raf/MEK/ERK and IKK/IκB/NF-κB signal cascades Biochemical Pharmacology. ,vol. 69, pp. 433- 449 ,(2005) , 10.1016/J.BCP.2004.10.006
Hitoshi NAGASHIMA, Hiroyuki NAKAGAWA, Keiko IWASHITA, Cytotoxic effects of nivalenol on HL60 cells Mycotoxins. ,vol. 56, pp. 65- 70 ,(2006) , 10.2520/MYCO.56.65
Yoshimitsu Oda, Sei-ichi Nakamura, Iwashiro Oki, Takesi Kato, Hideo Shinagawa, Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens Mutation Research\/environmental Mutagenesis and Related Subjects. ,vol. 147, pp. 219- 229 ,(1985) , 10.1016/0165-1161(85)90062-7
Yoshihisa Nozawa, Katsushi Nishihara, Richard M Peek, Motoko Nakano, Tatsuya Uji, Hirofusa Ajioka, Naosuke Matsuura, Hidekazu Miyake, Identification of a signaling cascade for interleukin-8 production by Helicobacter pylori in human gastric epithelial cells. Biochemical Pharmacology. ,vol. 64, pp. 21- 30 ,(2002) , 10.1016/S0006-2952(02)01030-4
Yoshio Ueno, Kiyoko Umemori, Eei-Chi Niimi, Sei-Ichi Tanuma, Satoshi Nagata, Masao Sugamata, Tomomi Ihara, Masaru Sekijima, Ken-Ichi Kawai, Ikuko Ueno, Fumio Tashiro, Induction of apoptosis by T-2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells. Natural Toxins. ,vol. 3, pp. 129- 137 ,(1995) , 10.1002/NT.2620030303
Tukumi Yoshizawa, Yin‐Zhe Jin, Natural occurrence of acetylated derivatives of deoxynivalenol and nivalenol in wheat and barley in Japan. Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment. ,vol. 12, pp. 689- 694 ,(1995) , 10.1080/02652039509374358
Shuji Tsuda, Yoko Kosaka, Makiko Murakami, Hiroaki Matsuo, Naonori Matsusaka, Kazuyuki Taniguchi, Yu F. Sasaki, Detection of nivalenol genotoxicity in cultured cells and multiple mouse organs by the alkaline single-cell gel electrophoresis assay Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 415, pp. 191- 200 ,(1998) , 10.1016/S1383-5718(98)00068-0
Franca Marino, Marco Cosentino, Anna Maria Fietta, Marco Ferrari, Simona Cattaneo, Giuseppina Frigo, Sergio Lecchini, Gian Mario Frigo, Interleukin-8 production induced by the endozepine triakontatetraneuropeptide in human neutrophils: role of calcium and pharmacological investigation of signal transduction pathways. Cellular Signalling. ,vol. 15, pp. 511- 517 ,(2003) , 10.1016/S0898-6568(02)00134-1
J RYU, The Acute and Chronic Toxicities of Nivalenol in Mice Toxicological Sciences. ,vol. 11, pp. 38- 47 ,(1988) , 10.1016/0272-0590(88)90268-0
Yoshitsugu Sugiura, Kazuaki Fukasaku, Toshitsugu Tanaka, Yukio Matsui, Yoshio Ueno, Fusarium poae and Fusarium crookwellense, Fungi Responsible for the Natural Occurrence of Nivalenol in Hokkaido. Applied and Environmental Microbiology. ,vol. 59, pp. 3334- 3338 ,(1993) , 10.1128/AEM.59.10.3334-3338.1993