Angiogenic characteristics of blood outgrowth endothelial cells from patients with von Willebrand disease.

摘要: BACKGROUND: Endothelial von Willebrand factor (VWF) inhibits angiogenesis. Accordingly, blood outgrowth endothelial cells (BOECs) isolated from disease (VWD) patients showed enhanced in vitro angiogenesis when compared with healthy control BOECs. Characterization of the angiogenic response VWD BOECs is limited and differences between different types have not been investigated detail. OBJECTIVES: The aim this study was to further explore potential pathogenic effect VWF mutations on METHODS: were four individuals, 10 one heterozygous carrier a type 2N mutation. Cell migration tube formation measured. RESULTS: Migration velocity total similar controls general. 3 patient 2B increased migratory other controls. Directional impaired eight out ability form tubes early passage numbers, but for CONCLUSION: can be useful tool ex vivo assessment cell function VWD, possible limitations, such as loss capacity, should recognized. most consistently impairment directionality migration. This first report properties BOEC, which