A Comparison of Methods for Quantifying Angiogenesis in the Matrigel Assay In Vitro

摘要: Angiogenesis is of major interest because its involvement in numerous pathologies or for promoting tissue repair. It often assessed by the ability endothelial cells to sprout, migrate, and form vascular tubules Matrigel vitro. contains a mixture basement membrane components, which stimulate capillary-like hexagonal structures, preferred over other vitro assays ease use, rapidity measure key steps angiogenesis, including migration, protease activity, tubule formation. Various methods have been used quantitate formation, yet no consensus has reached regarding best quantification method evaluating efficacy angiogenic stimulants inhibitors this assay. Here, we measured umbilical cord blood colony-forming cell-derived growth factor reduced presence absence two inhibitors, suramin SU6668, compare benefits limitations methods—Angiosys Wimasis. These comparative analyses revealed that both Angiosys Wimasis are easy accurately quantify will suit needs different types users.