The Impact of Proliferative Potential of Umbilical Cord–Derived Endothelial Progenitor Cells and Hypoxia on Vascular Tubule Formation In Vitro

摘要: Revascularization of the damaged tissue is pivotal to repair. Here, by bringing together two in vitro model systems, we have been able examine (1) ability human umbilical vein endothelial cells (HUVEC) containing a complete hierarchy progenitors derived from cord generate vascular tubules within stromal niche and (2) effects exposure low oxygen tensions on progenitor cell proliferation tubule formation vitro. Our results demonstrate that high proliferative potential colony forming (HPP-ECFC) cultured HUVEC preferentially contribute these are concentrated CD34(lo/-) fraction. were initially resistant when exposed hypoxia (1.5% O(2)) for short periods (1-2 days), but sustained chronic (4-14 days) inhibited their proliferate. This was reflected loss form cocultures dermal fibroblasts (hDFs). In contrast, an acute O(2) 24 h) followed reoxygenation did not adversely affect capacity both proliferate vitro.These studies therefore provide system study influences microenvironmental modification this HPP-ECFC.