Effects of obstetric factors and storage temperatures on the yield of endothelial colony forming cells from umbilical cord blood

摘要: As umbilical cord blood (UCB) is a rich source of endothelial colony-forming cells (ECFC), our aim was twofold: (1) to examine potential obstetric selection criteria for achieving the highest ECFC yields from UCB units, and (2) determine whether transient storage temperatures fresh cryopreservation units affected yield function. quality assessed before after by their clonogenic proliferative potential. Of 20 factors examined, placental weight only statistically significant factor that predicted frequency in UCB. Studies on effects revealed at 4°C reduced compared with 22°C, while MNCs significantly recoveries. To knowledge, this first demonstration temperature prior processing or culture have Our studies further support evidence compromises recovery.