Detection on polyacrylamide gel of a diagnostic nucleic acid from tissue infected with potato spindle tuber viroid

作者: T. J. Morris , N. S. Wright

DOI: 10.1007/BF02852039

关键词:

摘要: A diagnostic procedure for potato spindle tuber disease is described which allows detection of the causal viroid in small amounts and tomato tissue. The method involves extraction cellular nucleic acids, their separation by polyacrylamide gel electrophoresis staining with toluidine blue O. has been used to index greenhouse field potatoes mild severe strains pathogen

参考文章(9)
Milton Adesnik, Polyacrylamide Gel Electrophoresis of Viral RNA Methods in Virology. ,vol. 5, pp. 125- 177 ,(1971) , 10.1016/B978-0-12-470205-9.50010-1
K. H. Fernow, Tomato as a test plant for detecting mild strains of Potato spindle tuber virus. Phytopathology. ,vol. 57, pp. 1347- 1352 ,(1967)
T.O. Diener, Potato spindle tuber “virus” Virology. ,vol. 45, pp. 411- 428 ,(1971) , 10.1016/0042-6822(71)90342-4
R. P. Singh, Experimental host range of the potato spindle tuber ‘virus’ American Potato Journal. ,vol. 50, pp. 111- 123 ,(1973) , 10.1007/BF02857207
R. P. Singh, R. E. Finnie, R. H. Bagnall, Relative prevalence of mild and severe strains of Potato spindle tuber virus in Eastern Canada. American Journal of Potato Research. ,vol. 47, pp. 289- 293 ,(1970) , 10.1007/BF02863004
D. E. Hunter, H. M. Darling, W. L. Beale, Seed transmission of potato spindle tuber virus American Journal of Potato Research. ,vol. 46, pp. 247- 250 ,(1969) , 10.1007/BF02863021
Richard Stace- Smith, Eradication of Potato Spindle Tuber Virus by Thermotherapy and Axillary Bud Culture Phytopathology. ,vol. 60, pp. 1857- 1858 ,(1970) , 10.1094/PHYTO-60-1857