Effects of ultrasonic processing on caspase-3, calpain expression and myofibrillar structure of chicken during post-mortem ageing.

作者: Lin Chen , Xian-Chao Feng , Ying-yang Zhang , Xue-bo Liu , Wan-gang Zhang

DOI: 10.1016/J.FOODCHEM.2014.11.064

关键词:

摘要: In this investigation, ultrasonic treatments at a frequency of 40 kHz and power 1,500 W made caspase-3 calpain activities significantly higher in chicken muscle after slaughter during 5d storage (p<0.01). Additionally Western blotting analysis α-spectrin showed that caused the production degradation products 120 150 kDa more dense than control Degradation calpastatin meat ageing was induced by ultrasound (p<0.01), which suggested ability to cleave calpastatin. SDS-PAGE all enhanced accumulation 30 troponin-T product transmission electron microscopy images myofibrils displayed damage treatment for 60 min extensive min. The findings proved low-frequency high improved disruption myofibrillar structures increased proteolysis faster triggering an apoptosis cascade.

参考文章(36)
Tinghe Yu, Minghe Wu, Ping Huang, Lina Hu, Modulating Apoptosis in Cancer Therapy with Ultrasound and High-Intensity Nanosecond Electric Pulses Springer, Dordrecht. pp. 573- 590 ,(2013) , 10.1007/978-94-007-6211-4_22
Laurence Lagneaux, Eric Cordemans de Meulenaer, Alain Delforge, Marielle Dejeneffe, Martine Massy, Carine Moerman, Baudouin Hannecart, Yves Canivet, Marie-Françoise Lepeltier, Dominique Bron, Ultrasonic low-energy treatment: a novel approach to induce apoptosis in human leukemic cells. Experimental Hematology. ,vol. 30, pp. 1293- 1301 ,(2002) , 10.1016/S0301-472X(02)00920-7
Ahmed Ouali, Carlos Hernan Herrera-Mendez, Gerald Coulis, Samira Becila, Abdelghani Boudjellal, Laurent Aubry, Miguel Angel Sentandreu, Revisiting the conversion of muscle into meat and the underlying mechanisms Meat Science. ,vol. 74, pp. 44- 58 ,(2006) , 10.1016/J.MEATSCI.2006.05.010
Ming Huang, Feng Huang, Xinglian Xu, Guanghong Zhou, Influence of caspase3 selective inhibitor on proteolysis of chicken skeletal muscle proteins during post mortem aging Food Chemistry. ,vol. 115, pp. 181- 186 ,(2009) , 10.1016/J.FOODCHEM.2008.11.095
Robert W. Neumar, Y. Anne Xu, Hemal Gada, Rodney P. Guttmann, Robert Siman, Cross-talk between calpain and caspase proteolytic systems during neuronal apoptosis. Journal of Biological Chemistry. ,vol. 278, pp. 14162- 14167 ,(2003) , 10.1074/JBC.M212255200
Ming Huang, Feng Huang, Mei Xue, Xinglian Xu, Guanghong Zhou, The effect of active caspase-3 on degradation of chicken myofibrillar proteins and structure of myofibrils. Food Chemistry. ,vol. 128, pp. 22- 27 ,(2011) , 10.1016/J.FOODCHEM.2011.02.062
T.S. Awad, H.A. Moharram, O.E. Shaltout, D. Asker, M.M. Youssef, Applications of ultrasound in analysis, processing and quality control of food: A review Food Research International. ,vol. 48, pp. 410- 427 ,(2012) , 10.1016/J.FOODRES.2012.05.004
Fanbin Kong, Juming Tang, Mengshi Lin, Barbara Rasco, Thermal effects on chicken and salmon muscles: Tenderness, cook loss, area shrinkage, collagen solubility and microstructure Lwt - Food Science and Technology. ,vol. 41, pp. 1210- 1222 ,(2008) , 10.1016/J.LWT.2007.07.020
Naoki Hirokawa, Kazumitsu Koito, Futoshi Okada, Nobuki Kudo, Katsuyuki Yamamoto, Katsuhiko Fujimoto, Mutsumi Nishida, Takeshi Ichimura, Masakazu Hori, Taishi Satoh, Masato Hareyama, High-intensity focused ultrasound induced apoptosis with caspase 3, 8, and 9/6 activation in rat hepatoma Journal of Medical Ultrasonics. ,vol. 36, pp. 177- 185 ,(2009) , 10.1007/S10396-009-0234-2