Production and Quality Control of High-Capacity Adenoviral Vectors

摘要: Publisher Summary High-capacity adenovirus (HC-Ad) vectors have been developed to address capacity, toxicity, and immunogenicity problems of first- second-generation vectors. HC-Ad cannot be produced similar helper-independent vectors, in which most viral functions are provided from the vector. Since is a relatively large DNA virus that expresses many different protein RNA functions, it unlikely complementing cell lines can generated provide appropriate levels all “trans.” The DNA-based method described here allows for fast reliable determination three parameters with standard laboratory equipment independent or reporter gene expression. production process builds on two components; vector El-deleted helper packaging signal flanked by loxP sites serial amplifications producer cells expressing Crerecombinase. Excision results preferential genomes reduction contamination.