The host response to smallpox: Analysis of the gene expression program in peripheral blood cells in a nonhuman primate model

摘要: Smallpox has played an unparalleled role in human history and remains a significant potential threat to public health. Despite the historical significance of this disease, we know little about underlying pathophysiology or virulence mechanisms causative agent, variola virus. To improve our understanding pathogenesis variola-host interactions, examined molecular cellular features hemorrhagic smallpox cynomolgus macaques. We used cDNA microarrays analyze host gene expression patterns sequential blood samples from each 22 infected animals. Variola infection elicited striking temporally coordinated peripheral blood. Of particular interest were that appear represent IFN response, cell proliferation, immunoglobulin expression, viral dose-dependent patterns, modulation immune response. The virtual absence tumor necrosis factor α/NF-κB-activated transcriptional program face overwhelming systemic suggests products may ablate These results provide detailed picture response during infection, help guide development diagnostic, therapeutic, prophylactic strategies.