Slow skeletal muscle troponin T, titin and myosin light chain 3 are candidate prognostic biomarkers for Ewing's sarcoma.

摘要: Ewing's sarcoma (ES) is a common malignant bone tumor in children and adolescents. Although great efforts have been made to understand the pathogenesis development of ES, underlying molecular mechanism remains unclear. The present study aimed identify new key genes as potential biomarkers for diagnosis, targeted therapy or prognosis ES. mRNA expression profile chip data sets GSE17674, GSE17679 GSE45544 were downloaded from Gene Expression Omnibus database. Differentially expressed (DEGs) screened using R software limma package, functional pathway enrichment analyses performed enrichplot package GSEA software. NetworkAnalyst online tool, well Cytoscape its plug-ins cytoHubba NetworkAnalyzer, used construct protein-protein interaction network (PPI) conduct module analysis screen (hub) genes. LABSO COX regression overall survival (OS) Hub performed. A total 211 DEGs obtained by integrating analyzing three sets. functions pathways mainly associated with regulation small-molecule metabolic processes, cofactor-binding, amino acid, proteasome ribosome biosynthesis eukaryotes, Rac1, cell cycle P53 signaling pathways. one important 20 hub PPI Maximum Correlation Criteria algorithm cytoHubba. LASSO results revealed that titin (TTN), fast skeletal muscle troponin T, actin α-actin, nebulin, C type 2 (fast), myosin light-chain 3 (MYL3), slow T (TNNT1), myosin-binding protein C1 slow-type, tropomyosin heavy-chain 7 patients Kaplan-Meier curves demonstrated high levels TNNT1 (P<0.001), TTN (P=0.049), titin-cap (P=0.04), tropomodulin 1 (P=0.011), I2 (P=0.021) MYL3 (P=0.017) poor OS In conclusion, identified may be which, namely TNNT1, MYL3, prognostic