作者: Peter K. Howard , Janet Shaw , Anthony J. Otsuka
DOI: 10.1016/0378-1119(85)90011-3
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摘要: A 2.2-kb region of DNA containing the birA gene Escherichia coli has been sequenced. The sequence predicts a 35.3-kDal [321 amino acids (aa)] bifunctional protein biotin-operon-repressor and biotin-holoenzyme-synthetase activities. Mutations, generated by random insertion XhoI linkers, defined extent gene. Mutations affecting one or more five discernable properties [Barker, D. Campbell, A., J. Bacteriol., 143 (1980) 789-800] were mapped. Three mutations that result in temperature-sensitive (ts) growth, birA85, birA215, birA879 mapped N-terminal two-thirds protein. birA352 mutation, which partially complements birA215 birA879, maps third Finally, birA361 closest to terminus.