Mutant of Escherichia coli with Derepressed Levels of the Biotin Biosynthetic Enzymes

摘要: A cross-feeding technique was used to isolate a mutant of Escherichia coli K-12 that excretes 1,000 times more biotin into the growth medium than parent strain. The has high levels biosynthetic enzymes even when grown in presence biotin. Desthiobiotin synthetase, level which as measure activity pathway, is not repressed by at concentration 250,000 sufficient repress enzyme wild type. gene cotransducible with argC located 77 min on E. chromosome.