Blebs and apoptotic bodies are B cell autoantigens.

摘要: Mounting evidence suggests that systemic lupus erythematosus autoantigens are derived from apoptotic cells. To characterize the potential interactions between cells and B cells, D56R/S76R variant of 3H9, a murine autoantibody binds to DNA, chromatin, anionic phospholipids, was compared with DNA4/1, human anti-DNA autoantibody. Flow cytometry revealed only bound Jurkat treated either three distinct proapoptotic stimuli, Ab binding dependent on caspase activity, immunoreactivity developed subsequent annexin V binding. Confocal microscopy established structural basis for kinetics preferentially membrane blebs whereas did not require blebs. Inhibition ROCK I kinase, an enzyme stimulates nuclear fragmentation fragment distribution into blebs, significantly reduced Because members collectin pentraxin families serum proteins bind assist in clearance cellular remains, our results suggest Abs could affect recognition by innate immune system thus modify tolerance Ags.