3D elemental and structural analysis of biological specimens using electrons and ions.
作者： K. SCOTT
DOI: 10.1111/J.1365-2818.2010.03444.X
关键词:
摘要: Summary We demonstrate the utility of focused ion beam scanning electron microscopy combined with energy dispersive x-ray spectrometry for 3D morphological and elemental correlative analysis subcellular features. Although recent advances in super-resolution light techniques traditional transmission methods can provide cellular imaging at a wide range length scales, simultaneous features nanometre scale only be achieved such as capability. We technique by analysing silicon cell wall structure marine diatom, Thalassiosira pseudonana. This study also highlights limitations its current state suggests several possible improvements needed routine use biological specimens.
sci-hub.se 参考文章(29)
W.M. Darley, B.E. Volcani, Role of silicon in diatom metabolism Experimental Cell Research. ,vol. 58, pp. 334- 342 ,(1969) , 10.1016/0014-4827(69)90514-X
Joseph I. Goldstein, Dale E. Newbury, Joseph R. Michael, Nicholas W.M. Ritchie, John Henry J. Scott, David C. Joy, Scanning Electron Microscopy and X-Ray Microanalysis Transactions of the American Microscopical Society. ,vol. 102, pp. 59- ,(2018) , 10.1007/978-1-4939-6676-9
R. Buchanan, V. G. Macres, O. Preston, N. C. Yew, A New Scanning Electron Microscope Springer Berlin Heidelberg. pp. 341- 342 ,(1969) , 10.1007/978-3-662-12108-5_55
Manuel F Juette, Travis J Gould, Mark D Lessard, Michael J Mlodzianoski, Bhupendra S Nagpure, Brian T Bennett, Samuel T Hess, Joerg Bewersdorf, Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples Nature Methods. ,vol. 5, pp. 527- 529 ,(2008) , 10.1038/NMETH.1211
Monya Baker, Laser tricks without labels Nature Methods. ,vol. 7, pp. 261- 266 ,(2010) , 10.1038/NMETH0410-261
Jacques Rouquette, Christel Genoud, Gerardo H. Vazquez-Nin, Bernd Kraus, Thomas Cremer, Stanislav Fakan, Revealing the high-resolution three-dimensional network of chromatin and interchromatin space: A novel electron-microscopic approach to reconstructing nuclear architecture Chromosome Research. ,vol. 17, pp. 801- 810 ,(2009) , 10.1007/S10577-009-9070-X
Lucille A. Giannuzzi, Daniel Phifer, Nicholas J. Giannuzzi, Mario J. Capuano, Two-Dimensional and 3-Dimensional Analysis of Bone/Dental Implant Interfaces With the Use of Focused Ion Beam and Electron Microscopy Journal of Oral and Maxillofacial Surgery. ,vol. 65, pp. 737- 747 ,(2007) , 10.1016/J.JOMS.2006.10.025
Luciano G. Frigeri, Timothy R. Radabaugh, Paul A. Haynes, Mark Hildebrand, Identification of Proteins from a Cell Wall Fraction of the Diatom Thalassiosira pseudonana Insights into Silica Structure Formation Molecular & Cellular Proteomics. ,vol. 5, pp. 182- 193 ,(2006) , 10.1074/MCP.M500174-MCP200
Jurgen A.W. Heymann, Mike Hayles, Ingo Gestmann, Lucille A. Giannuzzi, Ben Lich, Sriram Subramaniam, Site-specific 3D imaging of cells and tissues with a dual beam microscope. Journal of Structural Biology. ,vol. 155, pp. 63- 73 ,(2006) , 10.1016/J.JSB.2006.03.006
Roman Schmidt, Christian A Wurm, Stefan Jakobs, Johann Engelhardt, Alexander Egner, Stefan W Hell, Spherical nanosized focal spot unravels the interior of cells Nature Methods. ,vol. 5, pp. 539- 544 ,(2008) , 10.1038/NMETH.1214